Research Interests

As part of the division’s mission, our goals are to incorporate the most up to date knowledge in the care of our patients. In order to provide this level of care and education we are involved with clinical/translational and basic science research.

Clinical/Translational Research:

North American Pediatric Renal Transplant Cooperative Study

Chronic Kidney Disease in Children (CKiDs) multicenter NIH funded trial

Prospective Pediatric Continuous renal replacement therapy multicenter trial

Midwest Pediatric Nephrology Consortium

Acute Dialysis Quality Initiative (www.ADQI.net) and the Acute Kidney Injury Network

Thrombocytopenia associated Multi Organ Failure trail center

Transplant Center Biobank Participating division

Multiple clinically focused and translational projects are ongoing- particular interests involving Acute Kidney Injury in Pediatrics

Laboratory Interests:

The Brophy laboratory focuses on the molecular genetics and functional mechanisms of normal and abnormal development of the kidneys and genitourinary (GU) tract. Laboratory interests focus on transcription factors and cellular signaling involved with mesenchymal to epithelial transition and metanephrogenesis, as well as identifying genetic lesions involved with selected renal diseases.Current projects include an NIH funded grant “Characterization of PAX2 Gene Targets, Binding Sites and Function, Nuclear Matrix and Chromatin” and a National Kidney Foundation of Michigan funded grant (completed) “Gene Identification in Mouse Congenital Progressive Hydronephrosis”. As a result of recent publications related to our work we have initiated and developed a collaborative genetics network (www.kidneygenes.com) in the areas of bilateral renal agenesis and progressive hydronephrosis which are conditions well known to the Pediatric Nephrology community. In addition we have developed an animal model system to test hypotheses related to these areas of research.

Potter’s Syndrome Genetics study (www.kidneygenes.com)

Congenital progressive hydronephrosis (www.kidneygenes.com)

Research Team /Lab Members:

• Jason Clarke Research Assistant III- Lab Manager
• Erik Bunchman BSc Research Assistant I
• Courtney Harkness Translational Research Assistant II
• David Segar- summer student
• Jeff Letolder- summer student

Research Affiliations:

• Department of Pediatrics, Division of Nephrology Genetics (in process)
• Molecular & Cell biology (in process)

Model System:

Animal models: predominantly mice, in vitro cell culture model systems, translational research-Human genetic biobanks and databases

Human clinical and outcomes trials (main focus Critical care based nephrology: Renal Supportive Therapies, Plasma exchange, hepatic dialysis and Extra-corporeal membranous oxygenation).

Recent Publications (past 3 years)

Stuart L. Goldstein Patrick D. Brophy, Jordan M. Symons, Michelle A Baum, Michael J.G. Somers, Timothy E. Bunchman. Outcomes in Pediatric CRRT: A Report of the ppCRRT Registry Group. Kidney Int. 2005 Feb;67(2):653-8.

McBryde KD, Bunchman TE, Kudelka TL, Pasko DA, Brophy PD. Hypertonic solutions in continuous renal replacement therapy for hyperosmolar acute renal failure. Pediatr Crit Care Med. 2005, 6(2):220-5.

Patrick D. Brophy, Jordan M. Symons, Timothy E. Bunchman, Michelle A Baum, Michael J.G. Somers,Stuart L. Goldstein. Anticoagulation in Pediatric CRRT: A Report of the ppCRRT Registry Group. Nephrol Dial Transplant. 2005, 20(7):1416-21.

Mills, DAM, White S, Flynn JT, Kershaw DB, Thomas SE, Brophy PD and Smoyer WE. Development and Implementation of Nursing Protocols. Nephrol Nurs J. 2005, 32(6):599-606.

Barletta JF, Barletta GM, Brophy PD, Maxvold NJ, Hackbarth RM, Bunchman TE.Medication errors and patient complications with continuous renal replacement therapy. Pediatr Nephrol. 2006, (6):842-5.

McBryde KD, Kershaw DB, Bunchman TE, Maxvold NJ, Mottes TA, Kudelka TL, Brophy PD.Renal replacement therapy in the treatment of confirmed or suspected inborn errors of metabolism. J Pediatr. 2006, 148(6):770-8.

Clarke JC, Honey EM, Bekker E, Snyman LC, Raymond RM Jr, Lord C, BrophyPD.A novel nonsense mutation in the EYA1 gene associated with branchio-oto-renal/branchiootic syndrome in an Afrikaner kindred. Clin Genet. 2006, 70(1):63-67.

Clarke JC, Patel SR, Raymond RM Jr, Andrew S, Robinson BG, Dressler GR, Brophy PD.Regulation of c-Ret in the developing kidney is responsive to Pax2 gene dosage. Hum Mol Genet. 2006

Stanley JC, Criado E, Upchurch GR Jr, Brophy PD, Cho KJ, Rectenwald JE; Michigan Pediatric Renovascular Group.  Pediatric renovascular hypertension: 132 primary and 30 secondary operations in 97 children. J Vasc Surg. 2006

Goldstein SL, Hackbarth R, Bunchman TE, Blowey D, Brophy PD. Evaluation of the PRISMA M10 circuit in critically ill infants with acute kidney injury: A report from the Prospective Pediatric CRRT Registry Group. Int J Artif Organs. 2006 Dec;29(12):1105-8.

Symons JM, Chua AN, Somers MJ, Baum MA, Bunchman TE, Benfield MR, Brophy PD, Blowey D, Fortenberry JD, Chand D, Flores FX, Hackbarth R, Alexander SR, Mahan J, McBryde KD, Goldstein SL. Demographic characteristics of pediatric continuous renal replacement therapy: a report of the prospective pediatric continuous renal replacement therapy registry. Clin J Am Soc Nephrol. 2007 Jul;2(4):732-8.

Cramer CH 2nd, Mills M, Valentini RP, Smoyer WE, Haftel H, Brophy PD. Clinical presentation and outcome in a cohort of paediatric patients with membranous lupus nephritis. Clinical presentation and outcome in a cohort of paediatric patients with membranous lupus nephritis. Nephrol Dial Transplant. 2007 Dec;22(12):3495-500.

Hackbarth R, Bunchman TE, Chua AN, Somers MJ, Baum M, Symons JM, Brophy PD, Blowey D, Fortenberry JD, Chand D, Flores FX, Alexander SR, Mahan JD, McBryde KD, Benfield MR, Goldstein SL. The effect of vascular access location and size on circuit survival in pediatric continuous renal replacement therapy: A report from the PPCRRT registry. Int J Artif Organs. 2007 Dec;30(12):1116-21.

Flores FX, Symons JM, Chua AN, Somers MJ, Baum MA, Bunchman TE, Benfield MR, Brophy PD, Blowey D, Fortenberry JD, Chand D, Hackbarth R, Alexander SR, Mahan J, McBryde KD, Goldstein SL. Continuous renal replacement therapy (CRRT) after stem cell transplantation. A report from the prospective pediatric CRRT Registry Group. Pediatr Nephrol. 2008 Apr;23(4):625-630.

Pinsky MR, Brophy P, Padilla J, Paganini E, Pannu N. Fluid and volume monitoring. Int J Artif Organs. 2008 Feb;31(2):111-26.

Betrus C, Remenapp R, Charpie J, Kudelka T, Brophy P, Smoyer WE, Lin JJ. Enhanced hemolysis in pediatric patients requiring extracorporeal membrane oxygenation and continuous renal replacement therapy. Ann Thorac Cardiovasc Surg. 2008 Dec;13(6):378-83.

Walters S, Porter C and Brophy PD. Dialysis and Pediatric acute kidney injury: Choice of Renal Support Modality. Peds Neph. In Press

Brophy PDRenal supportive therapy for pediatric acute kidney injury in the setting of multiorgan dysfunction syndrome/sepsis. Seminars in Nephrology. In Press

Bunchman TE, Goldstein SL and Brophy PD Technical Considerations for  Renal Replacement Therapy in Children Seminars in Nephrology. In Press

Email: patrick-brophy@uiowa.edu